Methyltrophic enzyme distribution in Methylosinus trichosporium
نویسندگان
چکیده
منابع مشابه
Analysis of methane biodegradation by Methylosinus trichosporium OB3b
The microbial oxidation of methane in the atmosphere is performed by methanotrophic bacteria that use methane as a unique source of carbon and energy. The objective of this work consisted of the investigation of the best conditions of methane biodegradation by methanotrophic bacteria Methylosinus trichosporium OB3b that oxidize it to carbon dioxide, and the use of these microorganisms in monito...
متن کاملPurification and properties of the methane mono-oxygenase enzyme system from Methylosinus trichosporium OB3b.
1. A three-component enzyme system that catalyses the oxidation of methane to methanol has been highly purified from Methylosinus trichosporium. 2. The components are (i) a soluble CO-binding cytochrome c, (ii) a copper-containing protein and (iii) a small protein; the mol. wts. are 13 000, 47 000 and 9400 respectively. The cytochrome component cannot be replaced by similar cytochrome purified ...
متن کاملProperties and partial purification of the methane-oxidising enzyme system from Methylosinus trichosporium.
The nature of the initial enzymic attack on methane by obligate methylotrophs has attracted considerable research effort in recent years. Strong evidence for oxygenase involvement came from 180 experiments which demonstrated that the oxygen in methanol, excreted by washed suspensions of Medzanomonas methanooxidans and Pseudomonas methanica, incubated under methane + oxygen mixtures was derived ...
متن کاملDetoxification of mercury by methanobactin from Methylosinus trichosporium OB3b.
Many methanotrophs have been shown to synthesize methanobactin, a novel biogenic copper-chelating agent or chalkophore. Methanobactin binds copper via two heterocyclic rings with associated enethiol groups. The structure of methanobactin suggests that it can bind other metals, including mercury. Here we report that methanobactin from Methylosinus trichosporium OB3b does indeed bind mercury when...
متن کاملGenome sequence of the obligate methanotroph Methylosinus trichosporium strain OB3b.
Methylosinus trichosporium OB3b (for "oddball" strain 3b) is an obligate aerobic methane-oxidizing alphaproteobacterium that was originally isolated in 1970 by Roger Whittenbury and colleagues. This strain has since been used extensively to elucidate the structure and function of several key enzymes of methane oxidation, including both particulate and soluble methane monooxygenase (sMMO) and th...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1975
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.122.2.433-436.1975